Flow cytometry optical filters are specialized components used in flow cytometers to separate, detect, and quantify different fluorescent signals emitted by labeled cells or particles. These filters ensure precise discrimination among various fluorescent dyes or markers, allowing accurate multi-parameter analysis in biological and medical applications such as immunology, cancer research, and cell sorting.
Model: Optical Filters
Specifications: Customized
Material: Optical glass
Key Types of Flow Cytometry Optical Filters
Bandpass Filters:
Allow only a specific wavelength range (or band) to pass through, blocking wavelengths outside of this range.
Typically described by a center wavelength (CWL) and a bandwidth (full width at half maximum, FWHM), such as a 530/30 filter, which transmits light centered at 530 nm with a ±15 nm range (515–545 nm).
Used for detecting specific fluorescent emissions by isolating each fluorophore’s emission peak.
Longpass Filters
Transmit light wavelengths longer than a specified cutoff, blocking shorter wavelengths.
For instance, a 600 nm longpass filter allows wavelengths above 600 nm to pass, isolating red or near-infrared emissions.
Often used to filter out excitation light, enhancing signal clarity for specific emissions.
Shortpass Filters
Transmit light wavelengths shorter than a designated cutoff, blocking longer wavelengths.
A 400 nm shortpass filter, for example, will allow only wavelengths below 400 nm to pass, helpful for UV and blue fluorescence applications.
Dichroic Filters (Dichroic Mirrors):
Reflect specific wavelengths while transmitting others, typically mounted at a 45-degree angle.
Separate excitation and emission light paths by reflecting excitation wavelengths and transmitting emission wavelengths.
Key for simultaneous multi-fluorescence detection, as they direct different fluorescent emissions to designated detectors.
Solution
| 方案一 ： | 方案二： | |||
| 488nm Filters（FWHM=10nm） | R488 T525 /575/615/680 Dichroic Mirrors | 661nm Filters（FWHM=20nm） | R661 T720 /780 Dichroic Mirrors | |
| 525nm Filters（FWHM=40nm） | R488/525 T575/615/680 Dichroic Mirrors | 720nm Filters（FWHM=20nm） | R661/720 T780 Dichroic Mirrors | |
| 575nm Filters（FWHM=31nm） | R488/525 /575 T615/680 Dichroic Mirrors | 780nm Filters（FWHM=60nm） | ||
| 615nm Filters（FWHM=25nm） | R488/525/575/615 T680 Dichroic Mirrors | |||
| 680nm Filters（FWHM=30nm） | ||||
Conventional size:
Narrowband filter: φ12.53.5mm (including retaining ring) Dichroic filter: 1313*1.1mm
Transmittance wavelength characteristics (reference data)
Application scenarios
Multi-color Fluorescence Detection: Filters allow simultaneous measurement of multiple fluorophores by isolating specific emission spectra, crucial for analyzing complex cell populations.
Signal Enhancement: Proper filter selection minimizes spectral overlap, reducing crosstalk and improving the signal-to-noise ratio.
Excitation and Emission Separation: Dichroic filters direct excitation light away from emission detectors, ensuring that detected signals originate solely from fluorescence emissions.
