Choose a germanium infrared window when you need a rugged, visible-blocking aperture for an 8 to 14 µm thermal imager that stays below roughly 100 °C. Choose a zinc selenide (ZnSe) window when the aperture has to pass a high-power CO2 laser beam at 10.6…
To match fluorescence filters to fluorophore spectra, do not select filters from excitation and emission peak wavelengths alone. Overlay the complete fluorophore excitation and emission curves with the excitation filter, dichroic and emission filter spectra, then evaluate how much useful spectral overlap is retained and…
Treat the three components as one optical system. To choose excitation, emission, and dichroic filters for fluorescence, start from the fluorophore’s absorption and emission spectra, place the dichroic edge in the crossover region between them, then set the exciter and emitter passbands so their combined…
